mouse embryonic fibroblast  (ATCC)

Journal: bioRxiv

Article Title: AI-discovered protein fragments as generalizable regulators of biomolecular condensates

doi: 10.64898/2026.05.08.723928

Figure Lengend Snippet: ( A ) Mouse embryonic fibroblasts (MEFs) were co-transfected with plasmids for expression of FAK 379-408 -2A-DsRed and FAK-GFP. After 24 hours the cells were seeded onto poly-D-Lysine plates and imaged for DNA (Hoechst), DsRed, and GFP fluorescence. ( B ) DNA, FAK 379-408 , and FAK imaging. Cells expressing significant FAK-GFP but not FAK 379-408 form condensates; cells expressing both FAK-GFP and FAK 379-408 exhibit drastic reduction of FAK condensates. ( C ) Representative cell expressing FAK-GFP only vs. cell expressing FAK-GFP + FAK 379-408 -2A-DsRed, at the same FAK transfection level (GFP intensity of ∼250 a.u.; Methods ). ( D ) Quantification of microscopy results. Number of FAK condensate puncta ( N condensates ) per cell as a function of the fragment:FAK (DsRed:GFP) intensity ratio in co-transfected cells. The number of puncta is negatively correlated with the intensity ratio (Spearman ρ = −0.54, P < 10 −10 ). ( E ) Bar plot from data in (D), comparing number of condensates per cell for fragment:FAK intensity ratios of ≤ 0.5 and ≥ 1. Bar height, mean value; error bars, standard deviation. *, difference is significant at p < 0.05, Student’s t-test.

Article Snippet: Mouse embryonic fibroblast (MEF ATCC CRL-2645) cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM; high glucose, Invitrogen) supplemented with 10% fetal bovine serum (FBS, Thermo Scientific), 1% penicillin–streptomycin, and 2 mM Glutamax at 37°C in a humidified incubator with 5% CO2.

Techniques: Transfection, Expressing, Fluorescence, Imaging, Microscopy, Standard Deviation

Journal: Materials Today Bio

Article Title: Feasibility of combining JAK1 gene editing via CRISPR-CasRx with EGCG–lactoferrin nanoparticle therapy in a microneedle-based platform for atopic dermatitis

doi: 10.1016/j.mtbio.2026.102884

Figure Lengend Snippet: Characterization and gene editing efficiency of PBAE-Plasmid NPs. ( a ) and ( b ) RNA silencing effects of different sgRNAs targeting JAK1 in NIH-3T3 and DC 2.4 cells. ( c ) Size and zeta potential of the PBAE-plasmid complex at various mass ratios. ( d ) Agarose gel electrophoresis of the PBAE/plasmid complex at different mass ratios. ( e ) Size distribution analyzed by dynamic light scattering (DLS) and transmission electron microscopy (TEM) images of PBAE-plasmid NPs at a mass ratio of 20:1. ( f ) and ( g ) Effects of the PBAE-plasmid complex at various mass ratios on NIH-3T3 and DC 2.4 cell viability. ( h ) Green fluorescence in NIH-3T3 cells transfected with PBAE-plasmid NPs. ( i ) JAK1 mRNA expression in NIH-3T3 cells transfected with PBAE-plasmid NPs. ( j ) JAK1 protein expression in mice transfected with PBAE-plasmid NPs. ( k ) Quantitative analysis of (j). Data are presented as mean ± SD (n = 3). Bars sharing the same letter are not significantly different, whereas those with different letters are statistically significant (p < 0.05).

Article Snippet: Mouse embryonic fibroblast NIH/3T3 cells and mouse dendritic DC2.4 cells were obtained from the American Type Culture Collection (ATCC).

Techniques: Plasmid Preparation, Zeta Potential Analyzer, Agarose Gel Electrophoresis, Transmission Assay, Electron Microscopy, Fluorescence, Transfection, Expressing